Conformational antigens and antibodies recognizing said antigens, a process for the efficient generation of monoclonal antibodies to native or conformational antigens expressed or carried by eukaryotic cells, a process for the selection of conformational antigens, use of monoclonal antibodies for therapeutical, diagnostic or vaccine applications

摘要

Conformational antigens and antibodies recognizing said antigens, a process for the efficient generation of monoclonal antibodies to native or conformational antigens expressed or carried by eukaryotic cells. A process for the selection of conformational antigens, use of monoclonal antibodies for therapeutical, diagnostic or vaccine applications. ;The process for preparing monoclonal antibodies, comprises: ;rendering an animal tolerant to an eukaryotic cell in a first state; ;detecting said tolerant animal; ;immunizing said tolerant animal, by injection of the eukaryotic cell in a second state carrying a neo-antigen or a non-self antigen; ;fusing B cells of said immunized mice with a myeloma cell line; and ;selecting the hybridoma expressing antibodies against said neo-antigen or non-self antigen.