GENERATION OF MONOCLONAL ANTIBODIES TO POORLY IMMUNOGENIC ANTIGENS EXPRESSED OR CARRIED BY EUKARYOTIC CELLS, USE OF MONOCLONAL ANTIBODIES FOR THERAPEUTICAL, DIAGNOSTIC OR VACCINE APPLICATIONS

摘要

Conformational antigens and antibodies recognizing said antigens, a process for the efficient generation of monoclonal antibodies to poorly immunogenic antigens expressed or carried by eukaryotic cells, use of monoclonal antibodies for therapeutical, diagnostic or vaccine applications. The process for preparing monoclonal antibodies comprises: (a) rendering an animal tolerant to an eukaryotic cell in a first state; (b) detecting said tolerant animal; (c) immunizing said tolerant animal, by injection of the eukaryotic cell in a second state carrying a neo-antigen non-self antigen; (d) fusing B cells of said immunized mice with a myeloma cell line; and (e) selecting the hybridoma expressing antibodies against said neo-antigen or non-self antigen.