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FABIAN AIMBRICATA RUIZ ET PAY. - ATTEMPTS FOR PROPAGATION AND PROTOPLAST CULTURE

机译:FABIAN AIMBRICATA RUIZ ET PAY。 -繁殖和原生质培养的尝试

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摘要

The paper presents results of experiments aimed at in vivo and in vitro propagation of Fabiana imbricata, an Andean evergreen shrub with impressive ornamental characteristics. Two ornamental types were studied: with white and with violet flowers. Bothin vivo and in vitro propagation methods were successful. The mean rooting of the cuttings was 87%, and in some cases reaching 100%, but the growing season influenced considerably the cuttings' production ability of donor plants. In the case of micropropagation, application of 0.2 mg I~(-1) BAP yielded more axillary shoots than higher BAP concentrations. The best regeneration of adventitious shoots from callus was obtained on medium with 1 mg I~(-1) BAP. Protoplast isolation was only possible from callus, because of the hard needle-shaped leaves of the species. The results proved that there are good opportunities for further breeding and use of this very promising ornamental in Europe.
机译:本文介绍了针对Fabiana imbricata(一种具有令人印象深刻的装饰特性的安第斯常绿灌木)在体内和体外繁殖的实验结果。研究了两种装饰类型:白色和紫色花。体内和体外繁殖方法均成功。插条的平均生根率为87%,在某些情况下甚至达到100%,但是生长期极大地影响了供体植株的插条生产能力。在微繁的情况下,施用0.2 mg I〜(-1)BAP产生的腋芽多于更高浓度的BAP。在含1 mg I〜(-1)BAP的培养基上,愈伤组织不定芽的再生效果最佳。由于该物种坚硬的针状叶片,原生质体只能从愈伤组织中分离出来。结果证明在欧洲有很好的机会进一步繁殖和使用这种很有前途的观赏植物。

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