Isolation of Outer Membrane Proteins (OMP) from Salmonella Cells Using Zwitterionic Detergent and Their Separation by Two-Dimensional Electrophoresis (2-DE)

摘要

We report that using the zwitterionic detergent Zwittergent Z 3-14 (R) to isolate outer membrane proteins (OMPs) from Salmonella O48 is suitable for their separation by two-dimensional electrophoresis (2-DE) in a capillary tube system. Sample preparation is a very crucial step for any bacterial proteomic study. Some modifications were introduced to the 2-DE protocol suggested by O'Farrell and BioRad, which significantly impaired the resolution of proteins. 2-DE analysis of OMPs may be helpful in the interpretation of the variable susceptibility of Salmonella O48 rods to the bactericidal activity of serum.