首页> 外文期刊>Chemical Senses >Maintenance of Rat Taste Buds in Primary Culture
【24h】

Maintenance of Rat Taste Buds in Primary Culture

机译:在原代培养中维持大鼠味蕾

获取原文
获取原文并翻译 | 示例
           

摘要

The differentiated taste bud is a complex end organ consisting of multiple cell types with various morphological, immunocytochemical and electrophysiological characteristics. Individual taste cells have a limited lifespan and are regularly replaced by a proliferative basal cell population. The specific factors contributing to the maintenance of a differentiated taste bud are largely unknown. Supporting isolated taste buds in culture would allow controlled investigation of actors relevant to taste bud survival. Here we describe the culture and maintenance of isolated rat taste buds at room temperature and at 37 deg C. Differentiated taste buds can be sustained for up to 14 days at room temperature and for 3-4 days at 37 deg C. Over these periods individual cells within the cultured buds maintain an elongaeted morphology. Further, the taste cells remain electrically excitable and retain various proteins indicative of a differentiated phenotype. Despite the apparent health of differentiated taste cells, cell division occurs for only a short period following plating, suggesting that proliferating cells in the taste bud are quickly affected by isolation and culture.
机译:分化的味蕾是一个复杂的末端器官,由多种具有不同形态,免疫细胞化学和电生理特性的细胞组成。单个味觉细胞的寿命有限,并经常被增生的基底细胞群体所替代。维持分化的味蕾的具体因素在很大程度上是未知的。在文化中支持孤立的味蕾将允许对与味蕾生存相关的演员进行受控调查。在这里,我们描述了在室温和37摄氏度下分离大鼠的味蕾的培养和维持。分化的味蕾在室温下可以维持长达14天,在37摄氏度下可以维持3-4天。在此期间,个体培养芽内的细胞保持伸长的形态。此外,味觉细胞保持电兴奋性并保留指示分化表型的各种蛋白质。尽管分化的味觉细胞具有明显的健康状况,但在铺板后细胞分裂仅发生了很短的时间,这表明味蕾中增殖的细胞会很快受到分离和培养的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号