The Negative Regulator OsSDS1 Significantly Reduces Salt and Drought Tolerance in Transgenic Arabidopsis

摘要

In this report, we present data on OsSDS1 (Oryza sativa L. salt and drought sensitive gene 1)-an uncharacterized gene isolated from rice Pei'ai 64S (O. sativa L.). Expression of OsSDS1 was strongly up-regulated by a wide spectrum of stresses, including cold, drought, and heat, in different tissues at different developmental stages of rice, as revealed by both microarray and quantitative RT-PCR analyses. Subcellular localization revealed that an OsSDS1: GFP fusion protein was distributed to the nucleus. Expression of OsSDS1 conferred decreased tolerance to salt and drought in Arabidopsis thaliana, accompanied by altered expression of stress-responsive genes and altered K+/Na+ ratio. The results suggest that OsSDS1 may act as a negative regulator of salt and drought tolerance in plants.