Isolation of pathogen/stress-inducible cDNAs from alfalfa by mRNA differential display.

摘要

Differential display of mRNA was used to isolate a full-length (SRG1) and a partial (SRG2) alfalfa (Medicago sativa) cDNA induced during infection with the fungal pathogen Colletotrichum trifolii. The deduced amino acid sequences were similar to each other and resembled plant defence-related proteins and tree pollen allergens. SRG1 is a member of a gene family in alfalfa which may also include the putative defence-related gene PR10. Unlike many defence-related genes described in similar systems, expression of SRG1-like genes does not correlate with resistance to C. trifolii. It is suggested that SRG1 is induced in response to plant stress. Nucleotide sequence data have been deposited in the EMBL/GenBank/DDBJ databases under accession numbers U42752 (SRG1) and U42753 (SRG2).