Salicylate and methyl jasmonate differentially influence diacetylene accumulation pattern in transformed roots of feverfew

摘要

In hairy root cultures of feverfew (Tanacetum parthenium (L.) Sch. Bip.), treated with methyl jasmonate (MJ), accumulation-of spiroketal enol ether type diacetylenes of known deterrent activity was increased about two-fold. The phenomenon was independent of the root clone studied. The maximum total acetylene content (approximate to 2.5 and 1.1% dry weight in clones M2 and I2, respectively) was observed after 72-96 h MJ treatment. Salicylic acid (SA) transiently reduced a content of constitutive spiroketal enol ethers, common for all root clones, but selectively enhanced accumulation Of cis-C-13-spiroketal enol ether epoxide ((E)-3,4-epoxy-2-(2,4-hexadiynylidene)-1,6-dioxaspiro[4.4]nonane) (2), present in clone M2. Simultaneous addition of SA,and MJ to the culture medium influenced diacetylene spectrum in a salicylate-like manner, but accumulation of the epoxide in clone M2 was more abundant (up to 6-fold increase compared with the control).