Applicability of the resistance gene - like fragment ADG2 as an RFLP probe in selection of extreme resistance to potato Y potyvirus (PVY)

摘要

Resistance gene-like fragment ADG2 has been shown to co-segregate with Ry_(adg), which controls extreme resistance to potato Y potyvirus (PVY) in Solanum tuberosum subsp, andigena (Hamalainen et al. 1997, 1998). Applicability tests of ADG2 as a selection tool for resistance to PVY were carried out by genomic Southern analysis on a broad range of cultivars and breeding lines of potato and some other solanaceous species. A new marker band (10.5 kb) associating with Ry was found in addition to previously reported 3.5 kb marker band. While the 3.5 kb marker was specifically linked to Ry_(adg) with extremely high association (96 %), the newly identified 10.5 kb marker band was detected in genotypes containing Ry_(adg) and other Ry genes. Some signals with ADG2 were also observed in other species within family Solanaceae inferring with the diversity of the corresponding chromosome region(s) among solanaceous species.