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Allozyme diversity of Russian wildrye accessions

机译:俄罗斯野马种的同工酶多样性

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Analysis of genetic diversity among plant accessions plays an important role in developing strategies for plant breeding and the conservation of genetic resources. Therefore, we sought to estimate the extent of genetic diversity present among and within 29 Russian wildrye accessions, which were collected from widespread geographic sites. We assessed the genetic diversity of eight enzymes found in 29 Russian wildrye accessions using vertical polyacrylamide gel electrophoresis. The genetic diversity of each accession was estimated from standard genetic parameters that included (1) the number of polymorphic loci; (2) the average number of alleles per locus; (3) the observed and expected heterozygosity. The eight enzymes were encoded by 13 putative loci and 46 alleles. Allozyme analyses revealed a large number of polymorphic loci among the 29 accessions and some of the accessions carry rare alleles and trace their origin to sites in Xinjiang, China, where Russian wildrye is indigenous. The accessions with rare alleles require additional evaluation to determine whether they possess unique phenotypic traits which would be useful in applied plant breeding.
机译:植物种间遗传多样性的分析在制定植物育种和遗传资源保护战略中发挥着重要作用。因此,我们试图估计从广泛的地理位置收集的29种俄罗斯野生黑麦种之间及其内部的遗传多样性程度。我们使用垂直聚丙烯酰胺凝胶电泳评估了29种俄罗斯野马种中发现的8种酶的遗传多样性。从标准遗传参数估计每个登录的遗传多样性,这些参数包括:(1)多态位点的数量; (2)每个基因座的平均等位基因数目; (3)观察到的和预期的杂合性。八个酶由13个推定基因座和46个等位基因编码。等位酶分析显示,这29个种质中有大量的多态性位点,其中一些种质携带罕见的等位基因,其起源可追溯到中国新疆的地方,而俄罗斯的野马是当地人。具有稀有等位基因的种质需要进一步评估,以确定它们是否具有独特的表型性状,这将在应用植物育种中有用。

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