Cytological and microbiological evaluation of uterine washing of bovine with reproductive problems (preliminary study)

摘要

Objective. Determination of the cytology and microbiology characteristics using low volume uterine lavage in dairy cows with reproductive problems in two herds on the cundiboyacence plateau. Materials and methods. 21 uterine lavage samples from cows with reproductive problems in two dairy herds were evaluated. The herds were located in Sibate (Cundinamarca) and Ventaquemada (Boyaca). 10 and 11 cows were sampled in each herd respectively. The uterine lavages were examined both macroscopically and microscopically. The cytological evaluation was done using Gram and Wright staining. The determination of the bacterial flora was done using standard microbiological methods used for the identification of the bacteria, the Kirby-bauer diffusion test for sensitivity was also done. Results. Bacilli, cocci, desquamating uterine epithelial cells (CEDU) and inflammatory response (IR) mediated by polimorphonuclear cells were observed. Bacterial isolates were Lactobacillus sp as part of normal floral (16.66%) and Klebsiella sp as accompanying flora (16.66%). There were also isolations of pathogenic bacteria that cause reproductive problems such as o Streptococcus sp. β hemolytic (33.33%), Streptococcus sp. α hemolytic (50%) y Streptococcus sp. γ hemolytic (50%) and Arcanobacterium pyogenes (Corynebacterium-Actinomyces pyogenes) (16.66%); spirochaete was observed in 9.52% of the samples. Conclusions. There was a direct relationship between macroscopic appearance of the uterine lavage, citology response and bacterial isolation.