Detection of Fluorophore-Tagged Recombinant Bovine Somatotropin (rbST) by Using a Silica-on-Silicon (SOS)-PDMS Lab-on-a-Chip

摘要

The presence of potentially harmful substances in milk is a concern for consumers. The discovery of recombinant DNA technology allowed the production of large quantities of recombinant bovine somatotropin (rbST), which is allowed to be used to increase milk and meat production in many countries. The use of rbST is controversial because of its potential effects on animal and human health. Use of the existing large instruments for the detection of rbST suffers disadvantages such as the need of large quantities of reagents, increased time of assays and most importantly, the high cost of equipment, etc. In this paper, a novel optical lab-on-a-chip (LOC) is proposed for the detection of a fluorophore-tagged rbST. The advantages of a silica-on-silicon platform for the optical waveguide and polydimethylsiloxane for microfluidics are exploited for the fabrication of a low-cost LOC. The tagging of rbST with two different types of fluorophores, such as FITC and Alexa-647, is carried out and used for detection in the proposed LOC.