An affordable, rapid determination of total lipid profile using paper-based microfluidic device

摘要

In this communication, a novel surface modification method of the paper surface using 3-aminopropyltriethoxysilane (APTES) and gold nanoparticles is reported. This functionalized paper (flower-shaped paper-based microfluidic devices (F-PMD) for simultaneous assay of multiple analytes) is used for determination of total lipid profiling (TLP) in a single device. Here, we have determined total cholesterol along with low-density lipoprotein (LDL), high-density lipoprotein (HDL) and triglycerides (TGL). The LDL and HDL were estimated by erasing the non-HDL and non-LDL components with a help of precipitating reagents. Standard graphs prepared by the present paper-based method nicely correlates with the conventional method (calculated by Roche-COBAS C111 autoanalyzer) with Pearson Correlation Coefficient (R) of 0.903, 0.9727, 0.9939 and 0.9866 respectively for total cholesterol, LDL, HDL and triglycerides. The present assay device is intended to assist in the screening of individual's lipid level for diagnosis of disorders involving high cholesterol level in the blood or for metabolic disorders associated with lipid and lipoprotein. As this is affordable, miniaturized, easily portable, self-testing and rapid, the present assay device can be used easily by pre-clinical, non-professionals, children in schools, labs, etc as a point-of-care diagnostics.