NUCLEIC ACID SIZE SELECTION

摘要

SPRIselect utilizes Solid Phase Reversible Immobilization (SPRI)-based chemistry to speed and simplify genomic DNA size selection for next generation sequencing fragment library preparation. Following shearing, the library construction process requires size selection to produce uniform distribution of fragments. SPRIselect allows size distribution to be adjusted between 150 and 800 base pairs to suit the application and sequencing platform. The process can be performed manually or automated for high throughput in 96-well plates. SPRIselect reagent kits are available in 5, 60, and 4S0 mL volumes, enable rapid and consistent size selection, and come with guidelines to assist users in customizing protocols. Gel cartridges, chips, and additional instruments are not needed.