Asymmetric somatic hybridization between Triticum aestivum L. and Haynaldia villosa Schur

摘要

Suspension cell-derived protoplasts of wheat, inactivated with different concentrations (0 - 2.5 mol/L) of IOA, were fused by PEG method with the Haynaldia villosa protoplasts which originated from the calli 4 - 5d after subculture and were irradiated with ~(60)Co-γ ray. Cell colonies, calli or regenerated plants were obtained from different combinations of fusion. The calli and plants were verified to be hybrids by chromosome counting, isozyme analysis and morphological inspection.