Glucose can reverse the effects of acute fasting on mouse ovulation and oocyte maturation

摘要

Food deprivation suppresses ovulation. Although nutritional elements are responsible for this suppression, itnis not clear whether energy metabolism has any effect on oocyte development under these circumstances. The aim of thenpresent study was to determine which nutritional element is responsible for the effect of acute fasting on mouse ovulationnand how oocyte development is affected. The results demonstrate that 64 h food deprivation blocks mouse ovulation.nThis was reversed by glucose feeding, oil feeding or short-term feeding, all of which elevated serum glucose levels.nFurthermore, 48 h food deprivation inhibited follicle-stimulating hormone-induced oocyte maturation in vitro. However,n48 h glucose feeding increased serum glucose levels and restored oocyte maturation. Food deprivation increased serumnprogesterone levels and decreased serum oestradiol levels. Food deprivation also impaired follicle development, caused thendeath of oocytes and attenuated glucose consumption by cumulus–oocyte complexes. Taken together, the results indicatenthat: (1) the suppression of ovulation by acute fasting may be due to the control of oocyte development; and (2) maintainingnserum glucose concentrations at a certain level is important for normal ovulation