Fabrication of branched polyethylenimin/alginic acid/poly(cyclohexane-1, 4-diyl acetone dimethylene ketal as a nano size carrier for controlled release of 5-fluorouracil

摘要

Self-assembly of nanoparticles (NP) with suitable sizes and surface charge to satisfy the functions of an efficient drug carrier plays a vital role in cancer therapy. Hence, the present study focused to prepare polymeric nanoparticles consisting of branched polyethylenimine (PEI), alginic acid (ALG) and poly(cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK), which were used to deliver an anticancer drug 5-fluorouracil (5-FU) to cancer cells. The size of the polymeric nanoparticles, measuring similar to 11 nm was confirmed by DLS and TEM analysis were revealed nanoparticles formation. Positive zeta potential of nanoparticles indicated that PEI forms the outer layer and the self-assembled of PEI, ALG and PCADK nanoparticles exhibited the fluorescence property. H-1 NMR and FTIR analysis confirmed the structural interactions between the PEI, ALG and PCADK during the formation of the nanoparticles. Drug release profiles across a 104-hour period comprising of both burst releases and sustained releases that resulted in 82%, 77% and 52% release of 5-FU at pH 5, 6.8 and 7.4 respectively. Cytotoxicity assay results showed that the 5-FU loaded nanoparticles exhibited greater cytotoxicity against WiDr colorectal adenocarcinoma cell lines compared to free 5-FU due to better adhesion to the cancer cells.