Myosin light chain kinase (MLCK) gene disruption in Dictyostelium: A role for MLCK-A in cytokinesis and evidence for multiple MLCKs

摘要

We have created a strain of Dictyostelium that is deficient for the Ca~2+/calmodulin-independent MLCK-A. This strain undergoes cytokinesis less efficiently than wild type, which results in an increased frequency of multinucleate cells when grown in suspension. The MLCK-A~- cells are able, however, to undergo development and to cap crosslinked surface receptors, processes that require myosin heavy chain.