UV endonuclease of Micrococcus luteus, a cyclobutane pyrimidine dimer-DNA glycosylase/abasic lyase: Cloning and characterization of the gene

摘要

The gene of Micrococcus luteus UV endonu- clease (cyclobutane pyrimidine dimer-DNA glycosylase/ abasic lyase) was cloned and characterized. The cloned gene, whose product had a predicted molecular mass of 17,120 Da, was found to be capable of complementing the Escherichia coli uvrA6 mutation in vivo with respect to resistance to acetone- mediated molecular photosensitization, a treatment produc- ing exclusively cyclobutane pyrimidine dimers in DNA. It also generated a nicking activity specific for photosensitization- treated DNA by in vitro transcription/translation.