NUTRIENT MEDIUM FOR CULTIVATION OF MICROCOCCUS LUTEUS VKPM V-2836 - A PRODUCER OF SITE-SPECIFIC ENDONUCLEASE MLUI

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 biotechnology and u043au0430u0441u0430u0435u0442u0441u00a0 u043fu043eu043bu0443u0447u0435u043du0438u00a0 site - specific endonuclease Mlu i, to u043fu043eu0432u044bu0448u0435u043du0438u00a0 way of biomass and enzyme u043fu0440u043eu0434u0443u0446u0435u043du0442u0430 site - sp u0435u0446u0438u0444u0438u0447u0435u0441u043au043eu0439 endonuclease Mlu i Micrococcus luteus wsc in - 2836 cultivate in the environment containing the following components, mas.%: u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442 u043au0430u0437u0435u0438u043du0430 1.8 - 2.0 - 3.0 2.7; yeast extract; sodium chloride, 0.2 - 0.3; u0434u0438u0441u0442u0438u043bu043bu0438u0440u043eu0432u0430u043du043du0430u00a0 water the rest. the advantage of the proposed environment u0437u0430u043au043bu044eu0447u0430u0435u0442u0441u00a0 increased the biomass and enzyme in 4 times compared to the cultivation of the known nutritional environment in the way biomass reaches 20 to 24 g of wet biomass with 1 l environment and activity of the enzyme - 100 - 420 thousand. iu / g, 2).(table 2). sl