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A mutation in the Cap Binding Protein 20 gene confers drought

机译:Cap Binding Protein 20基因中的突变赋予干旱

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摘要

In a genetic screen for Arabidopsismutants displaying pleiotropic alterations in vegetative development and stress responses we have identified a T-DNA insertion mutation in the Cap Binding Protein 20 (CBP20) gene, that encodes the 20kDa subunit of the nuclear mRNA cap binding complex (nCBC). Plants homozygous for the recessive cbp20 mutation show mild developmental abnormalities, such as serrated rosette leaves, delayed development and slightly reduced stature. Loss of the cbp20 function also confers hypersensitivity to abscisic acid during germination, significant reduction of stomatal conductance and greatly enhanced tolerance to drought. Expression of the wild type cDNA by CaMV35S promoter provides full genetic complementation of the pleiotropic cbp20phenotype. Phenotypic characteristics of the cbp20 mutant are very similar to those of recently described abh1mutant that is defective in the 80kDa subunit of nCBC. Our data thus confirm that both genes are dedicated to the same function. CBP20 provides a new target for breeding efforts that aim at the improvement of drought tolerance in plants. Our results also show that screening for pleiotropic phenotypes in mutant plant populations may be a fruitful strategy to isolate genes for agronomically important traits.
机译:在拟南芥突变体的遗传筛选中,该突变体在营养发育和应激反应中表现出多效性改变,我们已经确定了Cap Binding Protein 20(CBP20)基因中的T-DNA插入突变,该突变编码了核mRNA mRNA帽结合复合物(nCBC)的20kDa亚基。 。隐性cbp20突变纯合子的植物显示出轻度的发育异常,例如锯齿状的莲座叶,发育迟缓和身材略微降低。 cbp20功能的丧失也使发芽过程中对脱落酸的超敏性,气孔导度的显着降低和对干旱的耐受性大大增强。 CaMV35S启动子表达野生型cDNA提供了多效性cbp20表型的完全遗传互补。 cbp20突变体的表型特征与最近描述的在nCBC的80kDa亚基中存在缺陷的abh1突变体非常相似。因此,我们的数据证实了这两个基因都具有相同的功能。 CBP20为育种工作提供了新的目标,旨在提高植物的抗旱性。我们的研究结果还表明,筛选突变植物种群中的多效性表型可能是分离具有农学重要性状的基因的有效策略。

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