牦牛CAPN4基因启动子区突变对胴体及肉质性状的影响

摘要

为检测 CAPN4基因在牦牛群体中的多态性，分析 CAPN4基因启动子区突变对甘南牦牛胴体及肉质性状的影响，寻找与牦牛此类性状相关的分子遗传标记。采用 PCR-SSCP技术检测830头甘南牦牛、大通牦牛和天祝白牦牛CAPN4基因启动子区突变并分析其多态性，运用 SPSS 19.0软件中的 GLM 模型分析基因突变对甘南牦牛胴体及肉质性状的影响。牦牛 CAPN4基因启动子区存在 a.-1222G>A的单碱基突变，检测到 AA、AB 和 BB 3种基因型。相关性分析结果表明，a.-1222G>A的突变对不同年龄甘南牦牛胴体和肉质性状有一定影响，其中4，6岁牦牛 BB 型个体熟肉率显著高于 AA型或 AB型（PA突变影响不同年龄段甘南牦牛部分胴体及肉质性状，可作为甘南牦牛此类性状的遗传标记。%To search for the molecular markers associated with meat traits in yaks,CAPN4 mutations in the pro-moter region were investigated in different populations and their effects on carcass and meat quality were also ana-lyzed. A total of eight hundred and thirty yaks which sourced from Gannan yak,Tianzhu white yak and Datong yak were used in this study. PCR-SSCP method was used to detect the CAPN4 mutations in the promoter region. Associa-tions between mutations and the carcass and meat quality traits of Gannan yak were analyzed using the general line-ar model(GLM)in SPSS 19. 0 program. The mutation(a. -1222G >A)in the promoter region was identified, which led to three PCR-SSCP patterns( AA,AB and BB). The results of the statistical analysis indicated that the genotypes and allele effected carcass and meat quality in Gannan yak with different ages. At the ages of four and six,yaks possessing the genotype BB had higher cooking rate than those with possessing the genotype AA and AB (PA)in the promoter of CAPN4 had an effect on carcass and meat quali-ty partly,it might be used as a genetic marker to improve those traits in Gannan yak.