牦牛CAPN1基因intron 1多态性及其与胴体和肉质性状的关联性

摘要

The single nucleotide polymorphisms ( SNPs) of CAPN1 gene in Gannan yak, Tianzhu yak and Datong yak were detected by the technology of PCR-SSCP. The relationship between SNPs and Gannan yak carcass and meat quality traits was analyzed. The results of PCR-SSCP indicated that there was a genetic mutation of g. 100+606G>T in the first introns of CAPN1 gene of all 3 groups to form 3 genotypes (AA, AB and BB). Genetic analyses of CAPN1 gene showed that the polymorphism information contents in the detection region were 0. 25 T could be used as genetic markers.%采用PCR-SSCP技术检测甘南牦牛、天祝白牦牛及大通牦牛钙蛋白酶1（ CAPN1）基因单核苷酸多态性（ SNPs），并分析SNPs对甘南牦牛部分胴体及肉质性状的影响。结果表明，3类群牦牛在CAPN1基因第1内含子区检测到g.100＋606G＞T突变，形成了AA、BB和AB 3种基因型，检测区域表现为中度多态；CAPN1基因第1内含子突变影响不同年龄段甘南牦牛肌肉嫩度、失水率和眼肌面积，AA基因型个体嫩度剪切力值较低而眼肌面积较高，其中3岁和5岁牦牛AA型个体嫩度剪切力显著低于BB或AB型（P＜0.05），4岁和5岁牦牛AA型个体眼肌面积显著高于BB或AB型（ P＜0.05）；另外6岁牦牛AA型个体失水率显著高于BB和AB型（P＜0.05）。 CAPN1基因g.100＋606G＞T突变可用为甘南牦牛此类性状潜在的遗传标记之一。