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Differential Effects of Thiopental and Pentobarbital on Spinal GABAA Receptors

机译:硫喷妥钠和戊巴比妥对脊髓GABAA受体的差异作用

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摘要

General anesthetics thiopental and pentobarbital possess very similar chemical structures whereas their clinical potency is quite different. The underlying molecular mechanism is not fully understood. This study was designed to assess the differential effects of thiopental and pentobarbital on GABAA receptors of mechanically dissociated rat spinal dorsal horn neurons by using whole-cell patch-clamp technique. Pentobarbital, at a concentration of 30 μM, which markedly enhanced sub-saturated GABA-induced current (I GABA), had no effect on thiopental-induced maximal current. Similarly, the pentobarbital-induced maximal current was also not affected by 30 μM thiopental. Moreover, a linear summation of thiopental-induced maximal current and pentobarbital-induced sub-maximal current was observed. In addition, pentobarbital failed to further enhance I GABA in the presence of thiopental at a concentration with maximal modulatory effects on I GABA, and vice versa. Our results thus suggest that thiopental and pentobarbital might exert the GABA mimetic effects independently, but share a common mechanism to produce the GABA modulatory effects.
机译:全身麻醉药硫喷妥钠和戊巴比妥具有非常相似的化学结构,而它们的临床效价却大不相同。潜在的分子机制尚未完全了解。本研究旨在通过全细胞膜片钳技术评估硫喷妥钠和戊巴比妥对机械分离的大鼠脊髓背角神经元GABA A受体的差异作用。浓度为30μM的戊巴比妥显着增强了亚饱和GABA诱导的电流(I GABA ),对硫喷妥钠诱导的最大电流没有影响。同样,戊巴比妥诱导的最大电流也不受30μM硫喷妥钠的影响。此外,观察到硫喷妥钠诱导的最大电流和戊巴比妥诱导的亚最大电流的线性求和。此外,在硫喷妥钠存在的情况下,戊巴比妥不能进一步增强I GABA ,其浓度对I GABA具有最大的调节作用,反之亦然。因此,我们的结果表明,硫喷妥钠和戊巴比妥可能独立发挥GABA模拟作用,但具有共同的机制来产生GABA调节作用。

著录项

  • 来源
    《Neurochemical Research》 |2008年第10期|2159-2165|共7页
  • 作者单位

    Department of Neurobiology Medical College of Soochow University 199 Ren-Ai Road Suzhou Industrial Park Suzhou 215123 China;

    Department of Neurobiology Medical College of Soochow University 199 Ren-Ai Road Suzhou Industrial Park Suzhou 215123 China;

    Department of Neurobiology Medical College of Soochow University 199 Ren-Ai Road Suzhou Industrial Park Suzhou 215123 China;

    Laboratory of Cell Electrophysiology Wannan Medical College Wuhu 241001 China;

    Department of Neurobiology Medical College of Soochow University 199 Ren-Ai Road Suzhou Industrial Park Suzhou 215123 China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Thiopental; Pentobarbital; GABAA receptors; Mechanically dissociated neuron; Spinal cord dorsal horn;

    机译:硫喷妥钠;戊巴比妥;GABAA受体;机械分离的神经元;脊髓背角;

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