Detection of Homo- or Hetero-Association of Doks by Fluorescence Resonance Energy Transfer in Living Cells

摘要

The Dok proteins represent a family of adaptor proteins serving as common substrates for protein tyrosine kinases and play an important role in regulating signal transduction in multiple cell functions. Dimerization of Dok proteins may represent a powerful and flexible regulatory mechanism that can achieve a variety of consequences. This study aims to detect the homo- or hetero-association of Doks in living cells.