NMR analysis of budding yeast metabolomics: a rapid method for sample preparation

C. Airoldi; F. Tripodi; C. Guzzi; R. Nicastro; P. Coccetti

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NMR analysis of budding yeast metabolomics: a rapid method for sample preparation

机译：出芽酵母代谢组学的NMR分析：样品制备的快速方法

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Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by ~1H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.

机译：在这里，我们建议从酵母细胞中提取胞内代谢物及其1H-NMR谱图进行代谢谱分析的快速且可重复的方案的优化。通过比较处于不同生长阶段或具有不同遗传背景的细胞的代谢组，已验证了方案的可靠性。

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《Molecular BioSystems》 |2015年第2期|379-383|共5页
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C. Airoldi; F. Tripodi; C. Guzzi; R. Nicastro; P. Coccetti;
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Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy,SYSBIO, Centre of Systems Biology, P.zza della Scienza 2, 20126, Milan, Italy;

Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy,SYSBIO, Centre of Systems Biology, P.zza della Scienza 2, 20126, Milan, Italy;

Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy,SYSBIO, Centre of Systems Biology, P.zza della Scienza 2, 20126, Milan, Italy;

Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy,SYSBIO, Centre of Systems Biology, P.zza della Scienza 2, 20126, Milan, Italy;

Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy,SYSBIO, Centre of Systems Biology, P.zza della Scienza 2, 20126, Milan, Italy;

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NMR analysis of budding yeast metabolomics: a rapid method for sample preparation

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