Coupling process for plasma protein fractionation using ethanol precipitation and ion exchange chromatography

摘要

Blood is one of the most polluting by-products of the meat industries. Technologies have been developed to recover the proteins contained in blood, and in this study, a process coupling chemical precipitation with ion exchange chromatography was developed to separate and purify the main protein fractions of blood plasma. Ethanol was used as the agent for precipitation and Q-HyperD was used to purify the proteins by chromatography. To monitor all the steps and operations, analytical methods of electrophoresis and HPLC were stablished to identify the fractionated proteins. The results show that this process can recover the main plasma proteins with a high degree of purity for use in the pharmaceutical and food industries.