METROLOGICAL SUPPORT FOR DNA SEQUENCING

S. S. Golubev; S. A. Kononogov; Yu. A. Kudeyarov; A. V. Mardanov; P. Yu. Nikolaeva; N. V. Ravin; K. G. Skryabin

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METROLOGICAL SUPPORT FOR DNA SEQUENCING

机译：DNA测序的计量支持

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We discuss the operating principle of a pyrosequencer, which is an instrument for sequential reading of the nucleotide sequence of DNA molecules in pyrophosphate sequencing (pyrosequencing). We formulate the basic principles of metrological support for DNA sequencing and we present the results of studies of the metrological characteristics of the GS FLX pyrosequencer. We list the major problems encountered in designing a system for metrological support of DNA sequencing.

机译：我们讨论了焦磷酸测序仪的工作原理，焦磷酸测序仪是一种用于在焦磷酸盐测序（焦磷酸测序）中顺序读取DNA分子核苷酸序列的工具。我们制定了DNA测序的计量学支持的基本原理，并提出了GS FLX焦测序仪的计量学特性的研究结果。我们列出了设计用于DNA测序的计量支持系统时遇到的主要问题。

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来源
《Measurement techniques》 |2012年第3期|p.345-350|共6页
作者
S. S. Golubev; S. A. Kononogov; Yu. A. Kudeyarov; A. V. Mardanov; P. Yu. Nikolaeva; N. V. Ravin; K. G. Skryabin;
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作者单位

All-Russia Research Institute of Metrological Service (VNIIMS), Moscow, Russia;

All-Russia Research Institute of Metrological Service (VNIIMS), Moscow, Russia;

All-Russia Research Institute of Metrological Service (VNIIMS), Moscow, Russia;

Bioengineering Center, Russian Academy of Sciences, Moscow, Russia;

All-Russia Research Institute of Metrological Service (VNIIMS), Moscow, Russia;

Bioengineering Center, Russian Academy of Sciences, Moscow, Russia;

Bioengineering Center, Russian Academy of Sciences, Moscow, Russia;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
原文格式 PDF
正文语种 eng
中图分类
关键词
dna molecule; nucleotide sequence; complementarity; pyrophosphate sequencing (pyrose-quencing); reference material; plasmid Puc18;

机译：dna分子核苷酸序列互补性焦磷酸盐测序（葡萄糖测序）;参考资料;质粒Puc18;
入库时间 2022-08-17 13:11:10

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METROLOGICAL SUPPORT FOR DNA SEQUENCING

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