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首页> 外文期刊>Materials science & engineering >Osteogenic differentiation of mesenchymal stem cells (MSCs) induced by three calcium phosphate ceramic (CaP) powders: A comparative study
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Osteogenic differentiation of mesenchymal stem cells (MSCs) induced by three calcium phosphate ceramic (CaP) powders: A comparative study

机译：三种磷酸钙陶瓷（CaP）粉末诱导的间充质干细胞（MSCs）成骨分化的比较研究

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摘要

Calcium phosphate ceramics (CaPs) appear to be suitable alternatives for bone grafts because of their similarity to bone. Tricalcium phosphate (TCP), hydroxyapatite (HA) and biphasic calcium phosphates (BCP) are most commonly used CaPs. In this study, we studied and compared the osteoinductive ability of the powders of the three CaPs including HA, TCP and BCP (HA/β-TCP weight ratio of 60/40) by using bone marrow derived mesenchymal stem cells (MSCs). Osteoblastic formation, mineralization and gene expression of osteogenesis markers were measured in MSCs after 7, 14, 21 days of culture. Results showed a positive osteogenic differentiation effect of CaPs powders on MSCs as evidenced by an increased alkaline phosphatase (ALP) activity, positive ALP and Alizarin Red S staining, and upregulated osteoblastic gene expression compared with control. For the three CaPs, BCP powders showed the most prominent effect on osteoinduction. Next to BCP is TCP. Thus, this study supported that BCP may have better application prospects for repairing of bone defects in clinic.
机译：磷酸钙陶瓷（CaPs）由于与骨骼的相似性而似乎是骨移植的合适替代品。磷酸三钙（TCP），羟基磷灰石（HA）和双相磷酸钙（BCP）是最常用的CaP。在这项研究中，我们使用骨髓衍生的间充质干细胞（MSCs）研究并比较了三种CaP（包括HA，TCP和BCP）（HA /β-TCP重量比为60/40）的粉末的骨诱导能力。培养7、14、21天后，在MSC中测量成骨细胞的成骨形成，矿化和成骨标记的基因表达。结果表明，与对照相比，CaPs粉对MSC具有积极的成骨分化作用，这可以通过增加碱性磷酸酶（ALP）活性，阳性ALP和茜素红S染色以及上调成骨细胞基因表达来证明。对于这三种CaP，BCP粉末对骨诱导作用最显着。 BCP旁边是TCP。因此，这项研究支持BCP在临床修复骨缺损方面可能具有更好的应用前景。

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  • 来源
    《Materials science & engineering》 |2017年第11期|296-300|共5页
  • 作者

    Yuquan Li; Tongmeng Jiang; Li Zheng; Jinmin Zhao;

  • 作者单位

    Cuangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, The First Affiliated Hospital of Cuangxi Medical University, 530021 Nanning, China,Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Cuangxi Medical University, 530021 Nanning, China;

    Cuangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, The First Affiliated Hospital of Cuangxi Medical University, 530021 Nanning, China,Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Cuangxi Medical University, 530021 Nanning, China;

    Cuangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, The First Affiliated Hospital of Cuangxi Medical University, 530021 Nanning, China,Collaborative Innovation Center of Cuangxi Biological Medicine, The First Affiliated Hospital of Cuangxi Medical University, 530021 Nanning, China;

    Cuangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, The First Affiliated Hospital of Cuangxi Medical University, 530021 Nanning, China,Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Cuangxi Medical University, 530021 Nanning, China,Collaborative Innovation Center of Cuangxi Biological Medicine, The First Affiliated Hospital of Cuangxi Medical University, 530021 Nanning, China,Cuangxi Key laboratory of Regenerative Medicine, The First Affiliated Hospital of Cuangxi Medical University, 530021 Nanning, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Osteoinduction; Calcium phosphate ceramic powders; Mesenchymal stem cells;

    机译：骨诱导磷酸钙陶瓷粉;间充质干细胞;

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