ガスクロマトグラフィー質量分析による標準物質を必要としない乱用薬物一斉分析法の開発

摘要

近年わが国には，麻薬や覚せい剤，さらに類似の幻覚，rn麻酔作用があるにもかかわらず規制の対象となっていないrn多種多様な違法ドラッグがインターネットなどを介して急rn速に海外から流入し，これらの薬物の乱用は深刻な社会問rn題となっている．しかしながら原因薬物の特定が必要なrn法医鑑定や救急医療現場では迅速な薬物同定ができずに，rnその後の死因究明や患者の治療に多大な支障を釆たしていrnるのが実情である．%In this paper, we describe our approach to developing a systematic screening procedure for abused drugs that does not require the use of standard compounds. This procedure uses gas chromatography/mass spectrometry (GC/MS) with retention time locking. Fifty-five abused drugs, including amphetamine, piperazine, tryptamine and phenetylamine derivatives, opiates, and benzodiazepines, were selected as target compounds. The method consists of solid-phase extraction with a polar-enhanced Focus™ column followed by acetylation and GC/MS with retention time locking. Based on this method, an "abused drugs database," which includes the retention time, qualifier ion/target ion percentage, and calibration curve (values of slope and intercept), was constructed using the novel GC/MS software, NAGINATA™. Using this database, we found a significant improvement in the data analysis time, and qualitative and quantitative information about each drug was obtained without using standard compounds. The practical applications for examining urine samples in forensic cases are also presented.