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In Situ Imprinting of Topographic Landscapes at the Cell-Substrate Interface

机译:细胞-基质界面上的地形景观原位印记

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摘要

In their native environments, adherent cells encounter dynamic topographical cues involved in promoting differentiation, orientation, and migration. Ideally, such processes would be amenable to study in cell culture using tools capable of imposing dynamic, arbitrary, and reversible topographic features without perturbing environmental conditions or causing chemical and/or structural disruptions to the substrate surface. To address this need, we report here development of an in vitro strategy for challenging cells with dynamic topographical experiences in which protein-based hydrogel substrate surfaces are modified in real time by positioning a pulsed, near-infrared laser focus within the hydrogel, promoting chemical cross-linking which results in local contraction of the protein matrix. Scanning the laser focus through arbitrary patterns directed by a dynamic reflective mask creates an internal contraction pattern that is projected onto the hydrogel surface as features such as rings, pegs, and grooves. By subjecting substrates to a sequence of scan patterns, we show that topographic features can be created, then eliminated or even reversed. Because laser-induced shrinkage can be confined to 3D voxels isolated from the cell-substrate interface, hydrogel modifications are made without damaging cells or disrupting the chemical or structural integrity of the surface.
机译:在其原生环境中,贴壁细胞会遇到涉及促进分化,定向和迁移的动态地形线索。理想地,这样的过程将适合于使用能够施加动态,任意和可逆的地形特征而又不干扰环境条件或对底物表面造成化学和/或结构破坏的工具在细胞培养中进行研究。为了满足这一需求,我们在此报告了一种具有动态地形学经验的挑战性细胞体外策略的开发,其中通过在水凝胶中放置脉冲近红外激光焦点来实时修改基于蛋白质的水凝胶基质表面,从而促进化学反应交联导致蛋白质基质的局部收缩。通过动态反射掩模引导的任意图案扫描激光焦点,会产生内部收缩图案,该内部收缩图案会投射到水凝胶表面上,例如环状物,栓钉和凹槽。通过使基板经受一系列扫描图案,我们显示出可以创建,消除甚至颠倒地形特征。由于可以将激光引起的收缩限制在从细胞-基质界面分离的3D体素中,因此可以进行水凝胶修饰,而不会损坏细胞或破坏表面的化学或结构完整性。

著录项

  • 来源
    《Journal of the American Chemical Society》 |2018年第43期|14064-14068|共5页
  • 作者单位

    Univ Texas Austin, Dept Chem, Univ Stn A5300, Austin, TX 78712 USA;

    Univ Texas Austin, Dept Chem, Univ Stn A5300, Austin, TX 78712 USA;

    Univ Texas Austin, Dept Chem, Univ Stn A5300, Austin, TX 78712 USA;

    Univ Texas Austin, Dept Chem, Univ Stn A5300, Austin, TX 78712 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
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