首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >Molecularly imprinted electrochemical sensing interface based on in-situ-polymerization of amino-functionalized ionic liquid for specific recognition of bovine serum albumin
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Molecularly imprinted electrochemical sensing interface based on in-situ-polymerization of amino-functionalized ionic liquid for specific recognition of bovine serum albumin

机译:基于氨基官能化离子液体原位聚合的分子印迹电化学传感界面,用于牛血清白蛋白的特异性识别

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摘要

A molecularly imprinted polymer film was in situ polymerized on a carboxyl functionalized multi-walled carbon nanotubes modified glassy carbon electrode surface under room temperature. This technique provides a promising imprinting approach for protein in an aqueous solution using 3-(3-aminopropy1)-1-vinylimidazolium tetrafluoroborate ionic liquid as functional monomer, N, N'-methylenebisacrylamide as crossing linker, ammonium persulfate and N,N,N',W-tetramethylethylenediamine as initiator, and bovine serum albumin (BSA) as template. The molecularly imprinted polymerized ionic liquid film shows enhanced accessibility, high specificity and sensitivity towards BSA. Electrochemical sensing performance of the imprinted sensor was thoroughly investigated using K3Fe[CN](6)/K4Fe[CN](6) as electroactive probes. Under optimal conditions, the current difference before and after specific recognition of BSA was found linearly related to its concentration in the range from 1.50 x 10(-9) to 1.50 x 10(-6) mol L-1. The detection limit was calculated to be 3.91 x 10(-19) mol L-1 (S/N=3). The practical application of the imprinted sensor was demonstrated by determining BSA in liquid milk samples. (C) 2015 Elsevier B.V. All rights reserved.
机译:在室温下,将分子印迹聚合物膜原位聚合在羧基官能化的多壁碳纳米管修饰的玻璃碳电极表面上。该技术为使用3-(3-氨基丙基1)-1-乙烯基咪唑四氟硼酸根离子液体作为功能单体,N,N'-亚甲基双丙烯酰胺作为交联剂,过硫酸铵和N,N,N的水溶液提供了一种有前途的蛋白质印迹方法。以W-四甲基乙二胺为引发剂,以牛血清白蛋白(BSA)为模板。分子印迹聚合的离子液体薄膜显示出增强的可及性,高特异性和对BSA的敏感性。使用K3Fe [CN](6)/ K4Fe [CN](6)作为电活性探针彻底研究了印迹传感器的电化学传感性能。在最佳条件下,发现BSA特异性识别前后的电流差与其浓度在1.50 x 10(-9)到1.50 x 10(-6)mol L-1范围内线性相关。计算出的检出限为3.91 x 10(-19)mol L-1(S / N = 3)。通过测定液态奶样品中的BSA证明了印迹传感器的实际应用。 (C)2015 Elsevier B.V.保留所有权利。

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