Broadband Homonuclear Correlation Spectroscopy at High Magnetic Fields and MAS Frequencies

摘要

Structural studies of amyloid,membrane,and nanocrystalline peptides and proteins are currently being performed with high-resolution solid-state NMR,and the initial step in the investigation of these systems is assignment of the spectra.Spectral assignments of ~(13)C and ~(15)N backbone and side chain resonances,and subsequently ~(13)C-~(13)C and ~(13)C-~(15)N distance and torsion angle measurements,are accomplished by recoupling dipolar interactions during magic angle spinning (MAS) experiments,and a large number of broadband homonuclear recoupling techniques have been introduced which successfully accomplish this goal.