Emissive RNA Alphabet

摘要

A fluorescent ribonudeoside alphabet consisting of highly emissive purine (thA, thG) and pyrimidine ( thU, thC) analogues, all derived from thieno[3,4-d]-pyrimidine as the heterocyclic nucleus, is described. Structural and biophysical analyses demonstrated that the emissive analogues are faithful isomorphic nucleoside surrogates. Photophysical analysis established that the nucleo-sides offer highly desirable qualities, including visible emission, high quantum yield, and responsiveness to environmental perturbations, traits entirely lacking in their native counterparts.