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DNA-Mediated Assembly of Protein Heterodimers on Membrane Surfaces

机译：DNA介导的膜表面蛋白质异二聚体组装。

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摘要

We present a method based on self-assembling oligonucleotides to anchor proteins to a supported membrane surface. This anchoring method allows control of the surface density of multiple proteins. By incorporating additional recognition sequences into the DNA linkers, defined heterodimers can be produced upon the addition of a heterospecific DNA cross-linking strand. Characterization by fluorescence cross-correlation spectroscopy (FCCS) confirmed lateral mobility and the formation of specific heterodimers. We further demonstrate that proteins linked in this manner as either monomers or dimers can form functional interfaces with living cells.
机译：我们提出了一种基于自组装寡核苷酸的方法，将蛋白锚定到支持的膜表面。这种锚定方法可以控制多种蛋白质的表面密度。通过将额外的识别序列并入DNA接头中，可以在添加异源DNA交联链后产生确定的异二聚体。通过荧光互相关光谱法（FCCS）的表征证实了横向迁移率和特定异二聚体的形成。我们进一步证明以单体或二聚体的这种方式连接的蛋白质可以与活细胞形成功能性界面。

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  • 来源
    《Journal of the American Chemical Society》 |2013年第13期|5012-5016|共5页
  • 作者

    Michael P. Coyle; Qian Xu; Samantha Chiang; Matthew B. Francis; Jay T. Groves;

  • 作者单位

    Department of Chemistry University of California, Berkeley, California 94720, United States,Howard Hughes Medical Institute, University of California, Berkeley, California 94720, United States,Physical Biosciences and Materials Sciences Divisions, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States;

    Department of Chemistry University of California, Berkeley, California 94720, United States,Howard Hughes Medical Institute, University of California, Berkeley, California 94720, United States,Physical Biosciences and Materials Sciences Divisions, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States,Department of Physics, University of Illinois at Urbana-Champaign, 1110 West Green Street, Urbana, IL 61801;

    Department of Chemistry University of California, Berkeley, California 94720, United States;

    Department of Chemistry University of California, Berkeley, California 94720, United States,Physical Biosciences and Materials Sciences Divisions, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States;

    Department of Chemistry University of California, Berkeley, California 94720, United States,Howard Hughes Medical Institute, University of California, Berkeley, California 94720, United States,Physical Biosciences and Materials Sciences Divisions, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 入库时间 2022-08-18 03:12:31

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