MEPSET for separation and purification of the protein surface membrane selective enrichment and selective enrichment of the organic-inorganic composite surface membrane protein separation and purification how

摘要

MEPSET for the present invention is selectively enriched surface layer separation of protein purification (Membrane Protein Selective Enrichment and Topology) or absence group complex and the surface of the membrane protein separation and purification method for selective enrichment of that, more specifically, the surface membrane proteins of the optionally concentrated separated and purified for MEPSET (Membrane Protein Selective Enrichment and Topology) organic-inorganic composite is a host-guest chemistry and selectively enriched in the surface membrane separation and purification of the protein using organic-inorganic composite, a guest compound, the surface of membrane proteins coupled with, or the Guest compound and the surface membrane protein binding of the disulfide bonds linking functional group, wherein the guest compound with a host compound to bond, or a magnetic material coated with the host compound. The present invention many types of cells enriched for said surface membrane protein is mixed and pulverized with a certain concentration, separation, or purification therefore, surface membrane protein of a small amount is also easily removed, can be detected. Therefore, the glass on the surface of the membrane protein structure, active, discovery, expression, or function research, drug development, or is suitable for vaccine development. In addition, a recycling of expensive MEPSET magnetic particles, the surface membrane protein study is the advantage of the cost savings are.