Method of expressing a target protein on the surface of human cell membrane using the transmembrane anchored domain of signal transduction membrane protein

摘要

The present invention relates to a method of expressing a target protein on the surface of a human cell membrane using the transmembrane region of the signaling membrane protein, and more specifically, binding the transmembrane region of the signaling membrane protein to the C-terminus of the target protein and signaling When the fused target protein passes through the membrane by binding the peptide to the N-terminus of the target protein, the signal peptide is naturally cleaved and the transmembrane domain of the membrane protein is attached to the membrane, so that the target protein is expressed on the surface of human cell membrane. will be. The present invention has the feature of causing the expression of the fusion protein to occur temporarily by preparing the mRNA of the fusion protein and inserting it into human cells, so it is safe for clinical application of modified human cells, and the target protein is expressed on the surface of the cell membrane. Cells can enhance interaction with other human cells expressing a protein that can interact with a target protein on the cell surface.