Replicating the cross-sectional distribution of house-dust mite allergen found in carpet

摘要

With a view to producing carpets that could be used to determine the ease of particulate aerosolisation during domestic activity, we measured the cross-sectional distribution of dust-mite allergen, Der p 1, produced using American Society for Testing and Materials method (ASTM F608-89) for embedding house dust in carpets with that produced by several alternative protocols. Allergen concentrations produced at different levels within the pile using the different techniques were also compared with those in carpets from actual houses - in which the majority of allergen is typically found towards the base of the pile. To obtain profiles of allergen, horizontal sections, 2-mm thick, were taken from new carpets after they had been seeded with dust and embedded using one of four following techniques: (1) dragging a fixed roller across the surface of the carpet four times, (2) using the same roller but following it up with 200 revolutions in a hexapod wear simulator, (3) dragging the fixed roller across the carpet surface 30 times (the ASTM method), and (4) 2 minutes under a commercial plate compactor. Fibre from each 2-mm-thick section was collected and the Der p 1 content determined using enzyme linked immunosorbent assay and results expressed as ng Der p 1 per area in each section. Embedding with a fixed roller alone was not found to be particularly effective, resulting in roughly equal amounts of dust being apportioned within each pile layer, irrespective of the number of embedding passes used. In contrast, a distribution biased towards the base of the pile was found after roller-embedding/hexapod wear, although still to a lesser extent than has been observed in used carpets. Plate compaction gave a similar allergen distribution profile to combined roller/hexapod treatment but was considerably easier to perform. Thus, both techniques offer promise for researchers seeking to replicate the cross-sectional distribution of dust mite allergen found in carpets after actual use (and conceivably other particulate pollutants also).