FasL基因和屋尘螨过敏原基因共表达质粒DNA疫苗对屋尘螨致敏/激发小鼠气道炎症的作用

摘要

目的:观察FasL基因和屋尘螨过敏原Der p2基因共表达质粒的DNA疫苗(DNA-FasL-Der p2)对屋尘螨致敏/激发小鼠气道炎症的作用.方法:DNA-FasL-Der p2肌注接种屋尘螨致敏/激发C57BL/6小鼠,再次激发后处死小鼠.观察肺组织病理变化,检测支气管肺泡灌洗液(BALF)细胞总数和分类计数,以及IL-5、IFN-γ水平.并与仅编码Der p2基因的DNA疫苗(DNA-Der p2)比较.结果:接种DNA-FasL-Der p2和DNA-Der p2均能显著减轻屋尘螨致敏/激发小鼠气道炎症.减少BALF中细胞总数和嗜酸粒细胞比例.降低BALF中IL-5水平,以DNA-FasL-Der p2更显著.结论:DNA-FasL-Derp2可有效抑制屋尘螨致敏/激发小鼠气道炎症,而且比DNA-Der p2更有效.%Objective To investigate the effects of DNA vaccine encoding both FasL and house dust mite (HDM) allergen Der p2 (DNA-FasL-Der p2) on the airway inflammation in mice challenged with HDM.Methods HDM sensitized and challenged C57BL/6 mice were immunized with DNA-FasL-Der p2 or DNA-Der p2 (without FasL)DNA vaccine,then they were re-challenged with HDM.Two days after the last challenge, bronchoalveolar lavage fluid (BALF) was collected and lung histology was examined.The levels of IL-5 and IFN-γ in BALF supematants were analyzed by ELISA, total cell count and differential cell count in BALF sediment were determined.Results Both DNA-FasL-Der p2 and DNA-Der p2 relieved airway inflammation and decreased the level of IL-5, total cell count and the percentage of eosinophils in BALF.The improvement of DNA-FasL-Der p2 was more significant than that of DNA-Der p2.Conclusion DNA-FasL-Der p2 could reduce airway allergic inflammation in HDM-challenged mice.And DNA-FasL-Der p2 was more effective than that of DNA-Der p2.