The Chondrogenic Potential of Rabbit Bone-mar- row-derived Mesenchymal Stem Cells (MSCs) As- sayed under the Influence of Growth Factors

摘要

Conclusion Expression of chondrocytic phenotype by MSCs could be induced by the synergistic action of TGF-β_1, IGF- I and Vitamin C. Growth factor- induced MSCs could be a good cell source for tissue-engineered cartilage. Objective To establish a method of inducing rabbit bone marrow mesenchymal stem cells (MSCs) to express chondrocytic phenotype. Methods MSCs were seperated from bone marrow extracted from the iliac of New Zealand rabbit. TGF-β_1, IGF- I and Vitamin C were applied into culture medium to induce proliferation and transformation of MSCs. Colony forming efficiency ( CFE) was measured to reflect cell proliferation rate; procollagen α1 (II)mRNA in cells was detected by RT-PCR; ultrastructure of cells was observed under scanning electronic microscope (SEM); alkline phosphatase(ALP) in culture medium was also detected.