SIMULTANEOUS DETERMINATION OF METFORMIN HYDROCHLORIDE AND GLIPIZIDE IN TABLET FORMULATION BY HPTLC

摘要

Simultaneous determination of two antidiabetic drugs, metformin and glipizide, in pharmaceutical tablet formulations were investigated. Normal phase thin layer chromatography plate (silica gel 60 F₂₅₄) was used as the stationary phase and water:methanol:0.5% w/v ammonium sulfate solution (6:3:1.5 v/v/v) as the mobile phase to determine two pharmaceutically active ingredients in formulations of Glynase-MF. This system gave a good resolution for metformin (R_f value of 0.22 ± 0.01) and glipizide (R_f value of 0.85 ± 0.01). The λ_max was at 236 nm. The linear regression data for the calibration plot showed a good relationship with r = 0.9962 and 0.9930 for metformin and glipizide, respectively. The method was validated for precision and recovery. The limits of detection and quantification were 991.30 and 3003.95 ng/band for metformin and 9.57 and 29.01 ng/band for glipizide, respectively.View full textDownload full textKeywordsglipizide, HPTLC, metformin hydrochloride, oral antidiabetics, pharmaceutical analysisRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/10826076.2011.593227