Age Decline in the Activity of the Ca~(2+)-sensitive K~+ Channel of Human Red Blood Cells

摘要

The Ca~(2+)-sensitive K~+ channel of human red blood cells (RBCs) (Gardos channel, hIK1, hSK4) was implicated in the progressive densification of RBCs during normal senescence and in the mechanism of sickle cell dehydration. Saturating RBC Ca~(2+) loads were shown before to induce rapid and homogeneous dehydration, suggesting that Gardos channel capacity was uniform among the RBCs, regardless of age. Using glycated hemoglobin as a reliable RBC age marker, we investigated the age-activity relation of Gardos channels by measuring the mean age of RBC subpopulations exceeding a set high density boundary during dehydration. When K~+ permeabilization was induced with valinomycin, the oldest and densest cells, which started nearest to the set density boundary, crossed it first, reflecting conservation of the normal age-density distribution pattern during dehydration. However, when Ca~(2+) loads were used to induce maximal K~+ fluxes via Gardos channels in all RBCs (F_(max)), the youngest RBCs passed the boundary first, ahead of the older RBCs, indicating that Gardos channel F_(nax) was highest in those young RBCs, and that the previously observed appearance of uniform dehydration concealed a substantial degree of age scrambling during the dehydration process. Further analysis of the Gardos channel age-activity relation revealed a monotonic decline in F_(max) with cell age, with a broad quasi-Gaussian F_(max) distribution among the RBCs.