Oxymyoglobin Oxidation as Affected by Oxidation Products of Phosphatidylcholine Liposomes

摘要

Supernatants (105,000 × g) of oxidized phosphatidylcholine (PC) lipo- somes contained higher levels of thiobarbituric acid-reactive substances, and caused greater metmyoglobin formation when combined with oxy- myoglobin than those of controls (C) or PC plus tocopherol (PCT) (P<0.05). Products of PC liposome oxidation crossed dialysis mem- branes (mol wt cut-off = 500 Dalton) and accelerated the oxidation of oxymyoglobin more than C or PCT (P<0.05). The addition of known oxidation products of oleic acid and linoleic acid, fatty acids typical of PC, accelerated oxymyoglobin oxidation relative to controls (P<0.05). Results demonstrated that oxidation products of PC were prooxidative towards oxymyoglobin.