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首页> 外文期刊>Journal of Food Science >UHPLC/MS Identifying Potent α-glucosidase Inhibitors of Crape Pomace via Enzyme Immobilized Method
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UHPLC/MS Identifying Potent α-glucosidase Inhibitors of Crape Pomace via Enzyme Immobilized Method

机译:UHPLC / MS通过酶固定法鉴定绉果渣的有效α-葡萄糖苷酶抑制剂

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摘要

α-Glucosidases have been a major target in controlling and managing postprandial blood glucose and therefore diabetes treatment. This study aims to further identify and purify active compounds from the most active ethyl acetate fraction collected previously in Tinta Cao grape pomace extract (TCEE) using a newly developed and highly effective immobilization method, including obtaining compounds previously shown to inhibit the enzyme. Purification used crosslinked chitosan beads with α-glucosidases bound to polymer, which acted as immobilized enzyme vehicle to collect inhibitors. Compounds absorbed into the beads were eluded using methanol, where collected fraction was subjected to UHPLC-MS analysis to identify active compounds. Results presented 5 major compounds: viniferifuran (amurensin H), p-coumaroyl-6-O-D-glucopyranoside,p-coumaroyl-6-O-hexoside, (epi)catechin-hexoside, 10-carboxyl-pyranopeonidin 3-0-(6"-O-p-coumaroyl)-glucoside. These findings indicated the particular molecules can be utilized as potent α-glucosidases inhibitors, and may be further tested for postprandial glucose control.
机译:α-葡糖苷酶已成为控制和管理餐后血糖以及糖尿病治疗的主要目标。这项研究旨在使用一种新开发的高效固定化方法,从先前在田打草葡萄渣提取物(TCEE)中收集的最具活性的乙酸乙酯级分中进一步鉴定和纯化活性化合物,包括获得先前显示出可抑制该酶的化合物。纯化使用交联的脱乙酰壳多糖珠粒,其中的α-葡糖苷酶与聚合物结合,充当固定化酶载体以收集抑制剂。使用甲醇洗脱吸收到珠子中的化合物,然后对收集的馏分进行UHPLC-MS分析,以鉴定活性化合物。结果显示了5种主要化合物:viniferifuran(amurensin H),p-香豆酰基-6-OD-吡喃葡萄糖苷,p-香豆酰基-6-O-己糖苷,(epi)儿茶素-己糖苷,10-羧基-吡喃op啶3-0-(6 -Op-香豆酰基)-葡糖苷。这些发现表明,该特定分子可以用作有效的α-葡糖苷酶抑制剂,并且可以进一步测试餐后葡萄糖控制。

著录项

  • 来源
    《Journal of Food Science》 |2018年第6期|1131-1139|共9页
  • 作者单位

    Dept. of Nutrition and Food Science, Wayne State Univ., Detroit, Mich, 48202, U.S.A.;

    Dept. of Nutrition and Food Science, Wayne State Univ., Detroit, Mich, 48202, U.S.A.;

    Dept. of Nutrition and Food Science, Wayne State Univ., Detroit, Mich, 48202, U.S.A.;

    Dept. of Nutrition and Food Science, Wayne State Univ., Detroit, Mich, 48202, U.S.A.;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Chitosan beads; α-glucosidase inhibitor; immobilization; Tinta Cao grape pomace; UHPLC/MS;

    机译:壳聚糖珠;α-葡萄糖苷酶抑制剂;固定Tinta Cao葡萄果渣;超高效液相色谱/质谱;

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