Enzyme-immobilized electrode capable of easily immobilizing enzyme while maintaining activity, enzyme-immobilized electrode production electrode suitable for production of enzyme-immobilized electrode, and biofuel using this enzyme-immobilized electrode Provide batteries. In a biofuel cell having a structure in which a positive electrode and a negative electrode are opposed to each other via a proton conductor and configured to extract electrons from fuel using an enzyme, carbon particles are attached to at least one of the positive electrode and the negative electrode. An electrode made of a mixture containing a hydrophilic binder insoluble in water and having an enzyme immobilized thereon is used. Ketjen black or the like is used as the carbon particles, and ethyl cellulose or the like is used as the binder.
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