Development of Methods for the Recovery of Escherichia coli O157:H7 and Salmonella from Beef Carcass Sponge Samples And Bovine Fecal and Hide Samples

摘要

Culture methods were developed for the concurrent recovery of Escherichia coli O157:H7 AND Salmonella from bovine carcass, hide, and fecal samples. Several enrichment conditions were tested for the overall growth of pure cultures: tryptic soy broth for 2 h at 25 deg. C and then for 6 h at 42 deg. C was the protocol selected for use. Immunomagnetic separation (LMS) was incorporated for sensitivity and selectivity, along with a post-IMS enrichment for the recovery of Sabmonella as recommended by the manufacturer. Selective agars for plating after IMS were chosen on the basis of ease of target colony identification.