Optimizing Sporulation of Clostridium perfringens

摘要

Many sporulation media have been developed for Clostridium perfringens, but none stimulates sporulation for all strains. The aim of our experiments was to develop a sporulation method using Duncan and Strong (DS) medium, which supports Sporulation of a wide variety of strains. Different inoculation levels were tested, and the effects of sporulaiton-promoting Substances and acid shock were evaluated. Furthermore, DS medium was compared with other sporulation media. Highest Spore numbers in DS medium were obtained with a 10/100 24-h fluid thioglycollate broth inoculum (5.0×10~5/ml).