Three-solvent extracting method comprehensively evaluates phenolics profile and antioxidant activities of Tartary buckwheat

摘要

A three-solvent extracting method, which consist of 80% acetone, ethyl acetate, and70% ethanol extracting procedures, was adopted to evaluate phenolics profile andantioxidant activities of Tartary buckwheat. Phenolics profile of Tartary buckwheatwas determined using HPLC. Gallic acid, chlorogenic acid, and 2,3,4-trihydroxybenzoicacid were detected in 80% acetone extracts, 2,3,4-trihydroxybenzoic acid wasdetected in ethyl acetate extracts, rutin and quercetin were detected in 70% ethanolextracts, rutin was the dominant phenolic compound, and the contents ranged from1,902.0 to 2,402.8 mg/100 g DW. Antioxidant activities of Tartary buckwheat weredetermined using DPPH, ABTS, and FRAP assays, 70% ethanol extracts showed ahigher antioxidant activity than 80% acetone and ethyl acetate, respectively. Theseresults suggested that the three-solvent extracting method is more comprehensivethan independent one in evaluating phenolics profile and antioxidant activities ofTartary buckwheat.Practical applicationsThe three-solvent extracting method can be used to evaluate functional propertiesof Tartary buckwheat.