Chitin extraction from shrimp wastes by single step fermentation with Lactobacillus acidophilus FTDC3871 using response surface methodology

摘要

This study aimed to produce chitin and chitosan from shrimp wastes using lactic acidbacteria (LAB). From four strains of LAB and their mixture, Lactobacillus acidophilusFTDC3871 was selected due to higher proliferation and lactic acid production. Thefermentation parameters influencing on chitin and chitosan yields such as glucoseconcentration (w/w), inoculum size (v/w), fermentation time (h), and water content(mL) were optimized using response surface methodology (RSM) with central compositedesign (CCD). The optimum levels for glucose concentration, inoculum sizeand water content were 15% (w/w), 5% (v/w), and 100 ml after 72 hr of incubation at37°C. These conditions led to 90.8% of demineralization and 76% of deproteinizationwhich resulted in 57.7% of chitin yield. The highest concentrations of carotenoid extractedand lactic acid accumulated from hydrolysate were 183.9 μg/g and 21.3 g/L,respectively. Results showed potential application of L. acidophilus FTDC3871 in chitinextraction from shrimp waste.