Fluorescence-Enhanced Potassium Ions Detection Based on Inherent Quenching Ability of Deoxyguanosines and K+-Induced Conformational Transition of G-Rich ssDNA from Duplex to G-Quadruplex Structures

摘要

Herein, we develop a novel single fluorophore-labeled double-stranded oligonucleotide (OND) probe for rapid fluorescence-enhanced K+ detection, based on an inherent quenching ability of guanine bases and G-rich OND conformation transition from duplex to G-quadruplex. This probe presents high sensitivity and good selectivity for the detection of K+, and the assay process is simple and fast.